A study of the role of the reactive thiol group of rabbit muscle creatine kinase with a chromophoric reporter group.
نویسندگان
چکیده
Substrate- and ligand-induced conformational changes were studied in a series of thiol-modified derivatives of rabbit muscle creatine kinase that retained different amounts of enzymic activity. The results indicate that the 'reactive' thiol group of the enzyme is required for the conformational changes associated with formation of a 'transition-state analogue' complex.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 171 1 شماره
صفحات -
تاریخ انتشار 1978